English: Mouse DRG neurons were cultivated in microfluidic chambers in which the microfluidic flow is from left to right. This fluidic flow of the medium in the 5-micron-wide and 500-micron-long microgroove prevents diffusion of particles and allows a physical separation between cell bodies (left) and axon termini (right). CAV-2 particles covalently labeled with a fluorophore (Cy3) were incubated for 90 min in the axonal compartment before the video was started, and axons in the middle of the microgroove were imaged at one frame/second x 100 seconds.