English: In vivo ruffle induction in Vero cells by ASFV. Vero cells were serum-starved for 24 h and ASFV binding was allowed for 90 min at 4°C at MOI 100. After binding, images were collected for 30 min with an Orca R2 digital camera on a widefield microscope with controlled environmental chamber (temperature 37°C and 5% CO 2 humidified atmosphere). Images were captured at a resolution of 1344×1024 pixels using a 20×, 0.40 NA objective with a 1.6× magnification-changer.