English: Deoxyribonuclease (pyrimidine dimer) (EC 3.1.25.1) of wich the most studied is the endonuclease V of bacteriophage T4. The enzyme, possessed two distinct catalytic activities, a pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease. They acted in a sequential manner:

1) The glycosylase cleaved the N-glycosyl bond between the 5'-pyrimidine of a dimer and the corresponding sugar.

2) Then the endonuclease hydrolyzed a phosphodiester bond on the 3'-side of the apyrimidinic site.

Исходный код этого SVG-файла корректен.   Это structural formula было создано с помощью ChemSketch    Эта структурная формула использует встроенный текст для химических символов. This graphic of a chemical compound or chemical reaction was sketched in a freeware version of ChemSketch , with the Carbohydr. Res. (2003) structure drawing style. Then it was copied and pasted into Inkscape, and saved as .svg.

References

• Nakatsu Y, Nakabeppu Y, Sekiguchi M. (1982). "Action of T4 endonuclease V on polydeoxyribonucleotides with apyrimidinic or apurinic sites.". J. Biochem. 91 (6): 2057-65.
• Nakabeppu Y, Yamashita K, Sekiguchi M. (1982). "Purification and characterization of normal and mutant forms of T4 endonuclease V.". J. Biol. Chem. 257 (5): 2556-62.
• Dowd DR1, Lloyd RS. (1989). "Site-directed mutagenesis of the T4 endonuclease V gene: the role of arginine-3 in the target search.". Biochemistry. 28 (22): 8699-705..