English: Some endosomes complete the maturation process by splitting off a Rab5-positive endosome. Endosome enclosing a DENV particle (red, surrounded by a white circle) that splits off and leaves the Rab5-positive endosome (blue). The moment of splitting is indicated by the arrow, which points at the Rab5 endosome that is left behind. Real-time imaging and the preparation of the video are described in the legends to Videos S2 and S6 , respectively. During the time trace, the virus particle colocalizes with Rab7, but for clarity the signal is not depicted. Snapshots of the maturation event are shown in Figure 3D . The playback speed is 10× real-time.