English: Additional File 2 Peripheral deposition of GFP-APC puncta in COS-7 cells. Microtubule-associated GFP-APC puncta can be deposited at the cell periphery by shrinking microtubules. Two microtubules can be seen to grow towards the cell periphery depositing GFP-APC puncta at the cortex (arrowheads). Once the puncta has contacted the cell periphery the microtubule can be seen to shrink away from the cell edge leaving the original puncta at the periphery. Close observation of this image sequence suggested that GFP-APC puncta might remain associated with shrinking microtubule ends; this is examined in more detail in subsequent movies. Image capture rate was 1 frame/5s using a 63× oil immersion lens and the movie is played back at 20 frames/s.