English: Additional File 8 Cortically-localised GFP-APC in COS-7 cells. In confluent cultures of transfected COS-7 cells GFP-APC was present as a discontinuous array of punctate structures associated with membranes contacting adjacent cells. These puncta were far less dynamic than microtubule-associated GFP-APC distributions, being essentially immobile relative to the cell cortex. Image capture rate was 1 frame/10s using a 63× oil immersion lens and movie is played back at 20 frames/s.