English: Cytochalasin-B treatment interferes with Cav1 trafficking. Hela cells expressing Cav1-mRFP are put in suspension for 1 h replated on fibronectin-coated substrates for 3 h, incubated with 10 µg/ml cytochalasin-B for 30 min, and visualized using time-lapse Spinning Disk Microscopy. Images are taken each 2 s. Under these conditions, an accumulation of Cav1-mRFP positive vesicles appeared at the cell periphery.