English: Analyses of wild type RAD18 localization during the cell cycle. Time lapse images of nuclei expressing wild type YFP-RAD18. Images were captured every 30 min for 27 hours, a single nucleus was selected, aligned, and 5 frames of the images are shown per second. Endogenous RAD18 was downregulated by siRNA. Analyses began in S phase, and the cell went through G2, M and G1. The growth rates of wild type and mutant cells were not different (data not shown).