English: Additional File 3 Reversion of GFP-Nit1 granulation during the transmitted response An agarose mounted 35S GFP-Nit1 plant was wounded above the root meristem and imaged by the acquisition of Z-series at 2 minute intervals for 90 minutes. The wound site was above the field of view. By the time of acquiring the first time point, the granulation response had spread throughout much of the root meristem. Over time, the granules revert back to a cytoplasmic distribution pattern and the cells resume cytoplasmic streaming. Images are brightest point reconstructions of Z-series collected at 2 minutes intervals after wounding.