English: Additional File 4 Nuclear contraction during wound induced cell death The hypocotyl of an agarose imbedded nuclear marker line (N6) was wounded with a razor blade and them imaged at 2 minute intervals by collecting confocal Z-series. Propidium iodide was included in the imaging buffer to monitor plasma membrane integrity. Following wounding,, the nucleus on the left displays hypertrophy relative to the normal lentoid shape of unwounded cells (see the nuclei in cells on the right hand side in the movie). Over the course of the time series, the nucleus contracts concomitant with a decrease in GFP fluorescence, most evident in the time series between the 16 and 20 minute time points shown. This is followed later by intense staining of the contracted nucleus by propidium iodide.