English: Unanesthetized BLI. Imaging in unanesthetized mice was achieved by using a dedicated system that allows the simultaneous recording of both bioluminescence signal and a bright field video of the animal under infrared illumination for co-registration. This video shows an example of a mouse bearing 293T-Fluc cells at the neck region after the injection of D-luciferin. For quantification, the same large region of interest covering the whole area of movement was used for both unanesthetized and anesthetized imaging.