English: EGF does not affect microtubule plus-end-dynamics. Microtubule plus-ends were labeled by EB3-CFP in a MKN1 cell. The time-lapse sequence (10 frames/min) of fluorescence images (inverse presentation; left) and corresponding DIC images (right) was recorded first in the absence of EGF and then in the presence of 30 ng/ml EGF (corresponding Figure 3A, B). Note that EGF induces extensive ruffling and cell translocation that is coupled to retraction fiber formation at the rear. The associated substantial cell shape alterations necessitated adjustment of the focal plane resulting in a few out of focus images. Note also that plus-end-dynamics are not altered by EGF treatment.