English: Zyxin partially co-distributes with dSH2 in focal adhesions and is recruited from zyxin-enriched fibrillar adhesion upon EGF treatment. MKN1 were co-transfected with constructs encoding RFP-zyxin and CFP-dSH2 (depicted in false green color). Note that both co-localize in focal adhesions in the cell periphery but that the more centrally located focal adhesions and large fibrillar adhesions are predominantly positive for zyxin and contain very little dSH2 label. The video depicts a cell before and after addition of 5 ng/ml EGF (recording interval 50 s; see also corresponding Fig. 8D, D’). Note the reduction of the very large zyxin-positive plaque-like areas in the cell center and the appearance of novel contacts in newly-formed lamella (e.g., lower right). Bar, 5 µm.