English: PICs with mutant p12 proteins fail to dock to mitotic chromosomes. Interphase (A) and mitotic (B) cells of unsynchronized, PM14 GFP-infected U/R/RFP-H2A culture were visualized by time-lapse microscopy 12 hpi. The same procedure was applied to visualize mitotic cells of unsynchronized, PM14-infected NIH3T3/RFP-H2A culture (C); 2ME2-arrested U/R cells, co-infected with wt mCherry and PM14 GFP (D); and unsynchronized U/R/RFP-H2A cells infected with S(61, 65)A GFP (E) or with S(61, 65)A/M63I GFP (F). Broken lined circle marks chromosomal region with moving PM14 PICs with no apparent docking (B). Arrowheads point to representative wt mCherry PICs with stable chromosomal docking (D), or to an S(61, 65)A GFP PIC showing only transient, unstable association with the chromosomes (E). Frequency: 0.4, 0.4, 1.2, 0.8, 1.2 and 1.2 fps for A to F, respectively. Scale bar, 10 µm.