English: Additional data file 1 Movie1.mov – Real-time image sequence of an onion epidermal cell expressing GFP-MFP shows apparent interactions between peroxisomes and cortical MTs. Epifluorescence images (n = 200) were captured every 0.13 seconds. A mobile, dumbbell-shaped peroxisome (tracked with the green marker) appears to interact with MTs at several locations during the initial part of the image sequence before moving out of focus. Upon returning into the focal plane near the end of the image sequence, this peroxisome again appears to interact with MTs. The movement of this peroxisome is also shown in the fixed image sequence in Figure 6. Another peroxisome demonstrated a pausing event at a location where MTs were absent (tracked with the red marker), although it also paused several times at locations that coincided with MTs. The peroxisome tracked with the blue marker demonstrated on two occasions a subtle, lateral gliding movement alongside MTs. The cell observed in this image sequence was from an unpeeled epidermal layer that remained associated with the leaf segment.